Background and Objectives : With the increasing use of medicinal fungi and its effect on medical treatment, this branch of alternative medicine as antimicrobial additives have an important role in maintaining food quality. This study is aimed to determine the antimicrobial effects of aqueous and alcoholic extracts of macroscopic fungus Clitocybe brumalis.\nMaterial and methods: In this experimental study, during 2010-2011 (late fall and early winter in Zahedan and Khash), the stage of collecting the macroscopic fungus has been done, and then all samples have been examined in the microbiology lab of Islamic Azad University of Zahedan. At first, the bacteria were prepared from pure culture, and also the aqueous and methylen extracts have been prepared in the soxhelt apparatus. The effect of the concentrations of 1/0, 2/0 and 3/0 mg/mL were studied on bacteria Proteus volgaris, Salmonella typhimurium, and Staphylococcus epidermidis using the disk diffusion method, as well as minimum inhibitory concentration (MIC).\nResults: Certain concentrations of fungus extracts have antibacterial effects on some microorganisms. Although Effect of alcoholic extract was reduced by decreasing the concentration, the aqueous extract was reverse mode. The results indicated that aqueous and alcoholic extracts of Clitocybebrumalis contain compounds with anti-microbial properties. Study on Antimicrobial activity of macroscopic fungus extracts is a way to find new crucial combinations of microorganisms. \nDiscussion: indicated that the alcoholic extract effect (80% ethanol) of fugues Clitocybe brumalis on the bacteria Staphylococcus epidermises, Proteus volgaris and Salmonella typhimurium resulted in a clear halo. But in gram positive bacterial species diameters of halos at three different dilutions (0.1, 0.2 and 0.3) aqueous fungus extract haven\'t shown any antibacterial effect against gram-negative bacteria and also we noticed that the alcoholic extract has a direct effect on the bacterial species, while aqueous extract has less effect on the bacterial species.