The blocking effects of Eucalyptus plantation on Ageratina adenophora population and its allelopathic cause were studied in this paper with the combination of field investigation and indoor simulation experiment. The results showed: i) E. plantation had a certain blocking effect on the invasive A. adenophora population, mainly on the following three aspects: (a) the survival of A. adenophora plants. From the margin of E. plantation to its depth, the density of survived A. adenophora plants decreased, while the density of dead A. adenophora plants increased gradually; (b) the height of A. adenophora plants. The deeper it invaded in E. plantation, the shorter it became; (c) the biomass of A. adenophora plants. The biomass of those survived A. adenophora plants gradually decreased from the margin of E. plantation to its depth, while the biomass of those dead A. adenophora plants decreased in the beginning but increased later. ii) The chemicals in E. spp. showed a certain allelopathic activity towards the germination and seedlings growth of A. adenophora. Three different concentrations (25mg•L-1, 5mg•L-1, and 1mg•L-1) of such chemicals in E. spp. as 1,8-Cineole, α-Pinene, α-Terpineol, Limonene and Borneo showed an inhibitory effect on the germination rate (GR), germination index (GI) of A. adenophora seeds, and radicle and hypocotyl elongation of A. adenophora seedlings; while the synthetic effects on its germination and seedlings growth were featured with inhibitory effects, with the synthetic index (SI) of -0.47~-0.27, -0.38~-0.22, -0.27~-0.24, -0.41~-0.16, and -0.59~-0.18 for 1,8-Cineole, α-Pinene, α-Terpineol, Limonene and Borneo, respectively. The allelopathic activity of E. spp. was possibly the major cause for the blocking effects of E. plantation on the expansion of A. adenophora population. This study provided a new idea and a new way for the development and use of E. species and for the prevention and control of invasive weeds as well.