The species Aerides odorata Lour., a wild orchid native to the north and northeastern regions of Thailand, is likely to be to be depopulated due to the destruction and alteration of its natural environments, including over-collection of wild orchids. Even though germplasm conservation through traditional method is vital for maintenance of biodiversity and avoidance of genetic erosion, such method is time-consuming and difficult handle. Conversely, cryopreservation is an alternative to the traditional method to overcome the problem. The present study, therefore, aimed at establishing the protocol for the cryopreservation of A. odorata seeds by encapsulation-dehydration. The seeds were encapsulated in calcium-alginate before preculture in ND liquid medium supplemented with 0.5 M sucrose and maintained on a reciprocal shaker (100 rpm) at 25±2ºC under a long photoperiod (16 h light:8 h dark) with a photon dose of 40 µmol m-2 s-1 for 18 h.. The encapsulated seeds were then dehydrated by incubation in the sterile air flow of a laminar air flow cabinet for 0-6 h, and immediately plunged into liquid nitrogen (LN). After recovery from LN and rapid thawing in a waterbath (40 ºC), the germination rate and genetic alterations measured by flow cytometry were investigated. The highest germination rate (82%) was obtained from encapsulated seeds that were previously precultured in 0.5 M sucrose liquid medium for 18 h and sufficiently dehydrated for 6 h prior to storing in LN. Measured by flow cytometry, cryopreserved seeds did not show any evidence of genetic alterations.
The purpose of this study was to investigate the effectiveness of utilizing the multi-assessment strategy through a constructivist learning atmosphere with regard to perceptions of the prospective teachers. The participants were 98 third year (junior) prospective teachers attending to classroom management course in a public university in Turkey. Action research methodology and mixed method were utilized to collect data in this study. The results showed that classroom management field was acknowledged very positively by the most of the prospective teachers. The authentic activities utilized during the authentic instructions were positively recognized, although they admitted that all process was tiring and took long time. Although open ended questions yielded both positive and negative aspects, utilizing multi-assessment strategy was indicated mostly by the participants as highly effective. Findings indicated that employing constructivist assessment in teacher education may yield positive impacts especially when doing it learning by doing.
In general, lot of issue arises in hardware based on FPGA implementation. Today Elliptic curve cryptography had become trendier and many researchers concentrate on Elliptic Curve based on Power Analysis attacks, which exploits leakage information from the hardware devices. This paper concentrates on elliptic curve scalar doubling algorithm, which proposes certain rules and countermeasures for improving the performance and resistance against power analysis attack. The algorithms and their parameters were modified and assigned by the File Register Architecture, which is used to allot the registers in proper manner. The designing section uses Booth Multiplier and Carry Save Adder for increasing the efficiency and complexity of the algorithm. The implementation part uses Xilinx tool which belongs to Spartan III family in FPGA platform. The results were compared with the existing Elliptic Curve-Scalar Doubling algorithms, which was described by Yong Ki Lee and Lopez.
Salinity is one of the abiotic stress factors that negatively influence agricultural productivity and thereby affects world economy. It is a complex multigenic trait governed concurrently by many metabolic pathways. Detoxification of excess Reactive Oxygen Species (ROS), produced due to salt stress, by antioxidants is one of the strategies followed in living organisms to combat such stress. Manganese-Super Oxide Dismutase (Mn-SOD) is one such antioxidant metallo-enzymes, which plays a major role to scavenge ROS in diverse species. However, it is found that only few important residues of Mn-SOD enzyme neutralize excess ROS. Hence, the aim of this study is to identify the key residues of Mn-SOD that are actively involved in salt stress tolerance mechanism and that too conserved across species, through in silico proteomic analysis. In this study, protein sequences of Mn-SOD enzyme from diverse organisms associated with salt stress tolerance were collected from public domain and the conservancy of residues was studied both at sequence and structural level. The residues thus found to be conserved were reported as the key residues playing a significant role in salt stress tolerance mechanism in terms of contribution to the cofactor and substrate specificity, active site gateway formation and protein stability.
A solar still is viable option when demand of potable water does not exceed more than 3 liters. Enhancement in distillate output from the solar still is a main goal of many researchers in all over the world. In this research work, effect of using the copper and aluminium plates on distillate output is investigated experimentally as well as theoretically at different water depths under same climate conditions. In solar stills, first solar still augmented with copper plate, second with aluminium and third without any plate called passive solar still. An energy balance equations applied to solar still for calculation of theoretical distillate output of solar still with different plates. Three experimental still of 1 m2 each are constructed by locally available materials. In this research work, it is found that the results of experimental and theoretical results are in good agreement. It is also found that, using copper plate in solar still increases distillate output of 20 % (at water depth of 3 cm) and 32 % (at water depth of 6 cm) compared with passive solar still and using aluminium plate in solar still increases distillate output of 10% (at water depth of 3 cm) and 20 % (at water depth of 6 cm)
The theme of value creation, being the source of research, has increased interest in the field of management science, and hence became the new creed of managers. We have proposed the ethical behavior of managers influence the policy of value creation. In particular, we test the four cardinal virtues of the manager, who are the justice, prudence, strength and moderation, influence the development and growth of the company.\nThis paper attempts to make an inventory of ethical problems in a country sends development and clarify their role in the development of firms. We used a sample of Tunisian companies for a period to 2003-2011
In this dynamic scenario the communication no longer happens in predetermined manner. The network as a platform for communication comes with high infrastructure may likely to waste the resources. Thus, the ad hoc scenario networks came into existence. This network functionality has been enhanced through clustering mechanism. These clusters need to be perfect to sustain the efficient functionality of the network. Thus, this paper proposes F-PAC as a fuzzy logic based cluster validation technique to authenticate the cluster head identified by the W-PAC mechanism. This study has been shown using OMNET++ as simulator.
The life cycle assessment of Autoliv´s night vision camera shows higher global warming from use phase. This is because of energy use for the functioning of camera. The other major contribution of global warming is from transportation phase. This includes transportation of camera for installation and materials transportation for manufacturing of components. In this paper the transportation phase is discussed. If manufacturing of some components are transferred from America to Europe and camera is assembled in Europe instead of United States, the global warming percentage from transportation phase has been reduced from 17% to 7%.The material in camera that can be replaced/reduced for the environmental reasons is also discussed . Zinc and ceramic are found to be toxic from an environmental point of view. Finally, the environmental results by using electronic safety components and mechanical safety components in a vehicle are analyzed in this paper. The Mechanical safety components have more impacts than electronic components. But it depends upon choice of impact categories e.g. choosing global warming, eutrophication and eco toxicity, mechanical components have more impacts while with human toxicity and acidification, it is vice versa.